In this sixth article in the LabCoat Guide to BioStatistics series, intended as a basic refresher for scientists and technicians, we learn about Sample Size and Replication. Replication is the repeated application of treatments to multiple independently assigned experimental units. The number of independently assigned experimental units that receive the same treatment is the sample size. As variance increases for an experiment, it becomes more difficult to detect a significant difference, and a larger sample size will be required. In the absence of pilot trials or similar prior experiments, assumptions of CoV may be used together with Power and Effect Size for a rough estimate of required sample size when designing experiments. A biological rule of thumb is that a coefficient of variance of 20% or less is considered acceptable. #statistics #biostatistics #experimentalresearch #cropprotection #bioscience #GuideToEssentialBiostatistics #Experimentaldesign
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